Center for Biofilm Engineering
News Update:
October, 2005
Volume 8, Issue 10
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Research Highlights
Paper Spotlight
by Jeanne Olsen
Biofilms and biofouling in the oil industry
The chapter ‘Biofouling in the Oil Industry’ from Petroleum
Microbiology provides an excellent introduction to biofilms
including the formation, maturation, and interactions within the
biofilm itself and presents an overview of the complexity of
biofilms associated with the oil industry. The oil industry relies
on biofilms for several important processes including microbially
enhanced oil recovery (MEOR), oil spill degradation, bioremediation,
and bacterial production of chemicals such as xanthan gums,
biosurfactants, and enzymes. However, biofilms can also be
detrimental to the oil industry causing corroded pipelines,
reservoir souring and plugging, as well as
produced-water-injection-well plugging. Problems also exist with
current methods of biofilm control resulting from inappropriate
antimicrobial dosing protocols, development of antimicrobial
tolerance and resistance, and inadequate testing and monitoring. The
oil industry poses several research challenges including applying
laboratory studies to field situations. Developing laboratory
reactors that accurately represent field conditions is challenging,
as each industrial site has unique configurations. However, research
has been providing new solutions such as biochemical pathway
inhibition, inorganic compound inhibition, interspecies resource
competition, and improved dispersant technologies to minimize the
impact of biofilms in the oil industry.
“Biofouling in the Oil Industry”, Sanders, Peter F. and Paul J.
Sturman
In: Petroleum Microbiology, Bernard Ollivier and Michel Magot
(eds), Washington D.C., ASM Press, 2005. 171-198
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New Grant Awards
Thermal Biology Institute
Brent Peyton and Robin Gerlach have been awarded a grant through the
Thermal Biology Institute at Montana State University. The proposal
“Thermoalkaline Hydrolysis: Biotransformation of Organic Compounds at
High Temperature and pH,” was awarded $68,000/1yr, September 2005 −
2006. The practical application of this work will address the many
compounds of military, national security, agricultural, and
environmental significance that are more reactive at high temperature
and may be biodegradable under thermophilic conditions. The overall goal
of the project will be to look at both the fundamental and applied
aspects of organic biotransformation in geothermal systems.
AwwaRF and NSF
Anne Camper and her research team have been awarded three grants from
the American Water Works Association Research Foundation (AwwaRF), and a
fourth grant was awarded from NSF.
Synthesis Document on the State of Science of Molecular Techniques for
Application to the Drinking Water Industry. AwwaRF. $98,000/18 months
Anne Camper, PI, with Mark Burr and Andreas Nocker.
Microbiological Degradation of Haloacetic Acids (HAAs) in Distribution
Systems. Ray Hozalski, PI, University of Minnesota and Anne Camper,
co-PI, Montana State University. AwwaRF. $400,000/3 yrs.
Investigation of the Mode of Action of Stannous Chloride as an Inhibitor
of Lead Corrosion. Ray Hozalski, PI, University of Minnesota and Anne
Camper, co-PI, Montana State University. AwwaRF. $192,351/3 yrs.
Investing in People: Valuing a More Diverse Engineering Workforce. Susan
Capalbo, PI, and Anne Camper, Co-PI, Montana State University.
NSF/Gender in Science and Engineering (GSE)/ Small Grants for
Exploratory Research (SGER). $191,422/1 yr.
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New Industrial Associate Member
We are pleased to announce that NovaCal Pharmceuticals has joined
the Center’s Industrial Associate program. The designated
representative is Ron Najafi, Chairman and Executive Officer. Based
in Emeryville, CA, NovaCal Pharmaceuticals develops topical
anti-infective products that are safe for use when no other
anti-infective products should be used, such as for people with
diabetes and other disorders. Learn more about NovaCal
Pharmceuticals at their website:
http://www.novacal.com/index.html
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Recent CBE Publications
“Prevention of Staphylococcal Biofilm-Associated Infections by the
Quorum Sensing Inhibitor RIP”
Balaban, N., P. Stoodley, C.A. Fux, S. Wilson, J.W. Costerton, and
G. Dell'acqua
Clin Orthop Relat Res., (437):48-54 (2005)
“Impact of Ferrihydrite and Anthraquinone-2,6-Disulfonate on the
Reductive Transformation of 2,4,6-Trinitrotoluene by a Gram-Positive
Fermenting Bacterium”
Borch, T., W.P. Inskeep, J.A. Harwood, and R. Gerlach
Environmental Science & Technology 39(18):7126-7133 (2005)
http://dx.doi.org/10.1021/es0504441
“Ultrasonically Controlled Release of Ciprofloxacin from
Self-Assembled Coatings on Poly(2-Hydroxyethyl Methacrylate)
Hydrogels for Pseudomonas aeruginosa Biofilm Prevention”
Norris, P., M. Noble, I. Francolini, A. M. Vinogradov, P. S.
Stewart, B. D. Ratner, J. W. Costerton, and P. Stoodley
Antimicrobial Agents and Chemotherapy, 49(10):4272–4279
(2005)
To read paper abstracts or to order paper copies see:
http://www.erc.montana.edu/Res-Lib99-SW/pubs/Database/PD_DisplayScript.asp
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Education
Student Awards
Abbie Richards, a PhD candidate in Chemical and Biological Engineering,
received 3rd place and $250 at the INRA Subsurface Science Symposium
student poster competition. There were 34 posters and 6 finalists. Erin
Field, a PhD candidate in Microbiology was also a finalist.
Congratulations!
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CBE People in Action
Darla Goeres presented "Anti-bacterial Efficacy Against Surface
Bacteria Depends on the Method Used to Establish Bacteria on the
Surface" and Marty Hamilton presented "Surrogate Spore Study:
Statistical Results" to the Antimicrobials Division (AD), US-EPA, in
Arlington, VA, September 14, 2005. Darla and Marty met with AD science
teams and with the AD contract officer to discuss progress, planning,
and reporting for CBE projects funded by the AD, US-EPA.
Marty Hamilton also presented "Surrogate Spore Study: Statistical
Results" to the EPA Office of Pesticide Programs Microbiology Lab, in
Ft. Meade, MD, September 15, 2005, at a joint meeting of EPA scientists
and their collaborators from the US Army's Edgewood Chemical Biological
Center where the surrogate spore laboratory experiments were conducted.
Phil Stewart as an invited speaker presented "Controlling Microbial
Biofilms," in the Seminar Series at Center for Genomic Sciences,
Allegheny General Hospital, Pittsburgh, PA, September 19, 2005.
Robin Gerlach presented “Bacterial Transport and Biofilm Formation in
Porous Media,” Platform Presentation, INRA Environmental and Subsurface
Science Symposium, Big Sky, MT, September 19-21, 2005. Co-authors: J.P.
Gage, M. Adgie, J.D. Seymour, S.L. Codd, R.S. Sharp, A.B. Cunningham.
Erin Field presented a poster, “Influence of Carbon Source, Iron
Minerals, and Electron Shuttling Compounds on the Bacterial Reduction of
Chromate,” INRA Environmental and Subsurface Science Symposium, Big Sky,
MT, September 19-21, 2005. Co-authors: L.K. Jennings, A.B. Cunningham,
B.M. Peyton, R. Gerlach and W.A. Apel.
Abbie Richards presented a poster, “Characterization of a Siderophore
Produced by the Halo – Alkaliphile Halomonas campisalis,” INRA
Environmental and Subsurface Science Symposium, Big Sky, MT, September
19-21, 2005. Co-authors: B.M. Peyton (MSU), W.A. Apel (INL), A.K. Camper
(MSU) and J.N. Petersen (WSU).
Brent Peyton presented “Multiple Mechanisms of Uranium Immobilization by
Cellulomonas,” INRA Environmental and Subsurface Science Symposium, Big
Sky, MT, September 19-21, 2005. Co-authors: V. Sivaswamy, S. Viamajala,
R. Gerlach, W. Apel, R. Sani, and A. Dohnalkova.
Phil Stewart as an invited speaker presented "Diffusion, Dormancy,
and Detachment in Biofilms," to DuPont in Wilmington, DE, September 27,
2005.
Paul Sturman presented “In Vitro Reactor Systems for the Validation of
Anti-Biofilm Claims in Medical Devices," to the FDA, Washington, DC,
September 29, 2005.
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Biofilm Education Resources
Upcoming CBE Workshops
“Biofilm Methods Workshop,” organized by the CBE
Montana State University, February 1, 2006. See the sample 2005
agenda at:
http://www.erc.montana.edu/CBEssentials-SW/whats_new/sTAC05_Wrkshp.pdf
For more information about the Biofilm Methods Workshops, go to:
http://www.erc.montana.edu/Ind-Col99-SW/Workshops/
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Web Watch
BiofilmsOnline.com
See the BiofilmsOnline.com October issue at
http://www.BiofilmsOnline.com.
Subscribe to the bi-weekly newsletter by registering at:
http://www.biofilmsonline.com/cgi-bin/biofilmsonline/newsletter_signup.html
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__________________________________________
Diane Williams
(editor) of the CBE News Update
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