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Microscopy of Biofilms Workshop

July 14, 2008

 

Workshop participants and CBE instructors.


 

 

Special guest instructors were invited to share their expertise in microscopy.

 

 

Pictured:  Diane Gray, Molecular Probes™ - Invitrogen, Eugene, OR; and Thomas Neu, Helmholtz Centre for Environmental Research, Germany

 
 

 

 

 

A special thanks to Diane Gray, for providing the fluorescent stains and making this workshop such a success.

 

 


 

 

 

Betsey Pitts demonstrates how to stain biofilm covered coupons.

 

 


 

 

 

Kelli Buckingham-Meyer prepares biofilms for cryosectioning as Nancy Lin (NIST) and Kim Rafferty (Covidien) observe.

 

 


 

 

 

Thomas Neu discusses confocal microscopy images results with workshop participant, Gerald Hodgkinson.

 

Stains for this workshop were donated by Molecular Probes - Invitrogen.

THANK YOU TO.....

all the participants whose interest and participation made this such a great workshop.

 

Guest Instructors:

Diane Gray, Molecular Probes - Invitrogen

Thomas Neu, Helmholtz Centre for Environmental Research, Germany

 

CBE Instructors:

Alessandra Agostinho

Hans Bernstein

Kelli Buckingham-Meyer

Steve Fisher

Robin Gerlach

Lindsey Lorenz

Betsey Pitts

Liz Sandvik

Logan Schultz

Pat Secor

Diane Walker

 

Next Biofilm Methods Workshop


February 2, 2009
For information on upcoming workshops, see our Workshops page.

 

The Fluorescent Stains Used


 1. BacLight™ Live/Dead (Invitrogen #7012)

 2. FM® 1-43 (Invitrogen #T35356)
 3. FM® 4-64 (Invitrogen #T13320)
 4. Calcofluor™ White M2R (Invitrogen #L7009)
 5. Syto11 (Invitrogen #S7572)
 6. Syto 60
 7. Syto 62 (Invitrogen #S11344)
 8. Cell Mask Orange (Invitrogen #C10045)
 9. C2d (C2dichlorotriazine) (Invitrogen #T30200)
10. D2281 (m-dansylaminophenylboronic acid)
      (Invitrogen #D2281)
11. Sypro Ruby (Invitrogen #S12001)

12. Sybr Green (Invitrogen #S7563)

13. GSL1 - rhodamine

14. WGA - rhodamine

15. UEA I - rhodamine

16. LTL - fluorescin

17. STL-fluorescein

18. CTC (Invitrogen #34956)

19. Calcein AM green (Invitrogen #C3100MP)

20. Cell Tracker Green (Invitrogen #7025)

 

For more information about Molecular Probes™ - Invitrogen detection technologies contact:

Diane Gray
Ph: 541 465-8300 ext 59754
Fax: 541 335-0177

Email: Diane Gray  


 

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