Montana State University
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The summer 2010 Montana Biofilm Science & Technology Meeting (formerly TAC) for our Industrial Associate members and invited guests was held in Bozeman, MT, July 13-15, 2010 (Tuesday–Thursday). The Montana Biofilm Meeting featured sessions on ophthalmologic biofilms, wound care biofilms, biofilm fundamentals, biofilms in the built environment, systems analysis of biofilms, and biofilm methods. Please contact Paul Sturman (406) 994-2102 for more information. Click on title link to view complete article and agendas.

Have you ever wondered what statistical attributes define a standard disinfectant test?  In the third Knowledge Sharing Article, Professor Marty Hamilton discusses the eight statistical attributes: reasonableness, relevancy, validity, ruggedness, resemblance, responsiveness, repeatability, and reproducibility that a standardized disinfectant test should possess at an acceptable level.  Included in the discussion is the answer to one of the “top 10” frequently asked questions, “What are acceptable repeatability and reproducibility standard deviations?”

Heidi Smith, masters student in Land Resources & Environmental Science and the CBE, received a competitive NASA Earth and Space Science Fellowship (NESSF) for 2010/2011, with the possibility for two additional years of renewal support (30K each year).  Heidi’s project is entitled, “The role of microbes in microbial synthesis and transformation of dissolved organic matter in glacial environments.”  Heidi's advisor and mentor is Christine Foreman.

Michelle Olivo, ICU Medical, Dallas, Texas, and Marcia Ryder, consultant, Ryder Science, San Marcos, California, visited the CBE June 1-2, 2010.  They are working on on-going collaborations investigating needle-free devices and healthcare acquired infections with the Medical Biofilms Lab.

“The microbial fuel cell as an educational tool” 
Dewan A, Van Wie B, Beyenal H, Lewandowski Z
Chemical Engineering Education 2010; 44(2):157-165
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"Aerobic degradation of 2,4,6-trinitrotoluene by the yeast strain Geotrichum candidum AN-Z4"
Ziganshin AM, Gerlach R, Naumenko EA, Naumova RP
Microbiology 2010; 79(2):178-183
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Zbigniew Lewandowski taught several lectures on two general topics, “Mechanisms of biofilm processes,” and “Energy conversion in biofilms: Microbially influenced corrosion and microbial fuel cells,” Silesian University of Technology, Gliwice, Poland, May 8 – June 5, 2010.

Joe Seymour, as an invited speaker presented, “Transport in porous media: MR measurements and nonequilibrium statistical mechanics models of bio-reactive systems and colloid transport," Department Seminar Geophysics, Stanford University, Palo Alto, CA, May 12, 2010.

"Molecular characterization of the polymicrobial flora in chronic rhinosinusitis"
Stephenson MF, Mfuna L, Dowd SE, Wolcott RD, Barbeau J, Poisson M, James G, Desrosiers M
J Otolaryngol Head Neck Surg. 2010; 39(2):182-187
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“Production of cell–cell signaling molecules by bacteria isolated from human chronic wounds”
Rickard AH, Colacino KR, Manton KM, Morton RI, Pulcini E, Pfeil J, Rhoads D, Wolcott RD, James G
J Appl Microbiol, 2010; 108(5):1509–1522
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Trevor Zuroff was recently awarded a graduate research fellowship from the National Science Foundation. He will use the $90,000, three-year graduate research award to research using different organisms to break down cellulose. He will begin working on his doctorate at Penn State in the fall where he was also awarded a McWhirter fellowship granting him $90,000 for two more years after the NSF grant expires, plus $4,000 in research funds and funding to hire an undergraduate assistant.

"Anti-biofilm strategies and the need for innovations in wound care"
Ammons MCB 
Recent Pat Antiinfect Drug Discov. 2010; 5(1):10-17
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"Use of alternative carrier materials in AOAC official methodSM 2008.05, efficacy of liquid sporicides against spores of Bacillus subtilis on a hard, nonporous surface, quantitative three-step method"
Tomasino SF, Hamilton MA, Pines RM 
Journal of AOAC International 2010; 93(1):259-276
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We are pleased to welcome Agile Sciences as a new member company. Agile Sciences is a Delaware corporation headquartered in Raleigh, North Carolina. The company was founded in 2007 by Professors Christian Melander and John Cavanagh of North Carolina State University (NCSU) to provide commercial solutions to those industries plagued by the effects of biofilms. Agile Sciences' technology addresses problems associated with biofilm formation through the use of proprietary non-toxic organic compounds. The industrial representative will be Stephen R.

Isaac Klapper, Associate Professor of Mathematical Sciences, received the 2010 CBE Faculty Award  in May. He was given the award in grateful recognition of a decade of scholarly prominence in the field of biofilm modeling and productive interdisciplinary interaction with the CBE—including initiating and planning the 2007 Biofilm Mechanics Retreat.

Since May 2010, the CBE newsletter the Center for Biofilm Engineering has been publishing a series of articles written by Professor Marty Hamilton, CBE biostatistician. This series of Knowledge Sharing Articles is being written with the purpose of disseminating information that is the topic of presentations, posters, workshops or discussions that occur at the CBE.

Each Knowledge Sharing Article is a concise discussion on a specific topic. The information is not peer- reviewed, in the traditional sense, but these concepts have been debated, tested, refined and used at the CBE. Within one series, the terms and concepts will build on each other, similar to the way concepts are presented in a college course—so the articles are best read sequentially. These articles are a part of the CBE newsletter and are archived on the CBE web site.  Please click on title link to view the first of the series.

Dr. Anozie Ebigbo, Research Associate, Institute for Water Resources, University of Stuttgart, Germany, is visiting the CBE for 6 weeks. He is working with Dr’s Cunningham and Gerlach to develop simulation modeling capability which incorporates biofilm and biomineralization processes into an existing subsurface flow and reactive mass transport code which has been developed at the University of Stuttgart.

Ann Willis, CBE Technical Operations Manager, gave a tour of the CBE labs to a Japanese group with Yellowstone Glacier Adventures, Inc., on March 24, 2010. Students in this group had visited Yellowstone National Park and were eager to see how some of the organisms found in the hot springs environments are used in a variety of research projects at the Center.

Undergraduate and graduate students from all over campus presented their research projects at the annual celebration. With approximately 175 projects in all, they included the remote detection of meth labs, the effect of energy supplements on indoor rock climbing, coping strategies for college football players, gravitational waves, Russian movies, Yellowstone National Park microbes, and much more. There were several CBE associated students’ work presented and these are shown below.

Trevor Zuroff was the recipient of two fellowships. He was awarded the McWhirter Fellowship at Penn State University which will fund four years of graduate studies. He also received an NSF Graduate Research Fellowship which will fund three years of research and provide money for travel. Trevor will apply the NSF funding to study the optimization of biofilm reactors in bioprocess applications, while completing graduate coursework at Penn State. Trevor graduated from MSU May 8, 2010, with an undergraduate degree in chemical and biological engineering.

Phil Stewart will present, “Physiological activities and growth rate of Pseudomonas aeruginosa in biofilms revealed by transcriptional profiling,” American Society for Microbiology Meeting, San Diego, CA, May 26, 2010.

Erin Field, PhD candidate in microbiology, received the Ferguson Graduate Student Fellowship Award for her outstanding contributions both in research and in teaching. A generous contribution from an alumna of the Department of Microbiology generated this award. The recipient is chosen by the Graduate Curriculum Committee, and is based on the student’s contributions toward teaching and research.

“Effects of organic acids and heavy metals on the biomining bacterium: Acidithiobacillus caldus strain BC13”
Dissertation Defense by John Aston, Ph.D. Candidate in Chemical and Biological Engineering, Montana State University, March 2010
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“Influences of chemical speciation and solid phase partitioning on microbial toxicity: single organism to in situ community response"

"Real-time molecular monitoring of chemical environment in obligate anaerobes during oxygen Adaptive response"
Holman HYN, Wozeia E, Lin Z, Comolli LR, Ball DA, Borglin S, Fields MW, Hazen TC, Downing KH
Proceedings of the National Academy of Sciences 2009;106(31):12599–12604
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"Expression profiling of hypothetical genes in Desulfovibrio vulgaris leads to improved functional annotation"
Elias DA, Drury EC, Redding AM, Mukhopadyay A, B.Yen HC, Fields MW, Hazen TC, Arkin AP, Keasling JD, Wall JD