Montana State University

ASTM International Committee on Pesticides approves second biofilm standard

ASTM International  - Standards Worldwide
August 2007

Biofilm, self-organized communities of bacteria that can have both positive and negative effects in a variety of industries, is the subject of a new ASTM International standard, E 2562, Test Method for Quantification of Pseudomonas aeruginosa Biofilm Grown with High Shear and Continuous Flow Using CDC Biofilm Reactor. The new standard is under the jurisdiction of Subcommittee E35.15 on Antimicrobial Agents, which is part of ASTM International Committee E35 on Pesticides and Alternative Control Agents.

MSU biofilms research helps set standards for everyday products

by Michael Becker, MSU News Service
August 22, 2008

Montana State University scientist Darla Goeres knows that there is more than one way to grow a biofilm, a fact that she uses to make sure that when a product claims it kills "99 percent" of bacteria, it really does the job.

Standardized Biofilm Method Approved

Three standardized biofilm methods now provide researchers with a choice of unique reactor systems for growing biofilm in the laboratory.

Biofilm Standard Methods News - April 2009

Darla Goeres attended the ASTM E35.15 subcommittee meeting held in Vancouver, BC, Canada, on April 20–22, 2009. The purpose of the meeting was to standardize methods used for medical and industrial microbial testing. At this meeting, Darla was appointed to the position of Recording Secretary for the ASTM E35 Main Committee.

A New Series: Knowledge Sharing Articles on Standard Biofilm Methods

Have you ever wondered what statistical attributes define a standard disinfectant test?  In the third Knowledge Sharing Article, Professor Marty Hamilton discusses the eight statistical attributes: reasonableness, relevancy, validity, ruggedness, resemblance, responsiveness, repeatability, and reproducibility that a standardized disinfectant test should possess at an acceptable level.  Included in the discussion is the answer to one of the “top 10” frequently asked questions, “What are acceptable repeatability and reproducibility standard deviations?”

A New Series: Knowledge Sharing Articles on Standard Biofilm Methods

Following the steps listed in a standard method enables a researcher to achieve the desired outcome. Similarly, the development of a method also follows a process to achieve the desired outcome of standardization. This process is described in the fourth Knowledge Sharing Article titled, “Testing surface disinfectants: Method development phases.”

Read the next in series,"Testing surface disinfectants: Method development phases"(KSA-SM-04.pdf)

A New Series: Knowledge Sharing Articles on Standard Biofilm Methods

The development of new methods for testing disinfectants is a multi-disciplinary enterprise. It is tempting for physical scientists and engineers to apply evaluation criteria that are widely-used in their disciplines. This article explains why disinfectant test methods require different evaluation strategies and acceptance criteria than used in the physical sciences.

A New Series: Knowledge Sharing Articles on Standard Biofilm Methods

When estimating the number of bacteria in a sample, it is a common practice in microbiology to use viable plate counts. In this Knowledge Sharing Article, Al Parker and Marty Hamilton present the formulas, examples and justification for pooling the counts from more than one dilution, even when the counts are below the acceptable range, to form the best possible estimate of the true number of bacteria in the sample.

A New Series: Knowledge Sharing Articles on Standard Biofilm Methods

The log reduction measure of disinfection efficacy was introduced in the second Knowledge Sharing Article.  Although the log reduction (LR) is an important facet of quantitative disinfectant test methodology, only a succinct definition was provided in KSA-SM-02.  The purpose of this article is to present calculation formulas, some interesting properties of the LR, a discussion the relationship between LR and two other popular measures of disinfectant efficacy (the survival fraction and the percentage killed), and procedures for calculating both the LR and the within-experiment standard devi

Now Available: Ninth in a series of Standardized Methods papers

April 7, 2011
This article focuses on the importance of checking whether harvesting and disaggregating steps bias the results of a surface disinfectant test.

Industry news: ASTM Committee E35 approves fourth biofilm method

April 22, 2011

Method E2799-11, titled “Standard Test Method for Testing Disinfectant Efficacy against Pseudomonas aeruginosa Biofilm using the MBEC Assay,” was approved during the April 2011 E35 committee meeting in Anaheim, CA.

CBE standardized methods lab presents series of articles on testing surface disinfectants

September 19, 2011
The mission of the CBE is to advance the basic knowledge, technology, and education required to understand, control and exploit biofilm processes.

ASTM committee approves fifth biofilm method

May 2, 2012

Method E2871-12, titled “Standard test method for evaluating disinfectant efficacy against Pseudomonas aeruginosa biofilm grown in the CDC biofilm reactor using the single tube method,” was approved by ASTM Committee E35 during the April 2012 committee meeting in Phoenix, Arizona.

Now available: Thirteenth Knowledge Sharing Article (KSA) on standardized methods

September 13, 2013

KSA-13: Using R to assess resemblance, repeatability, and reproducibility for quantitative and semi-quantitative disinfectant methods

Now available: Fourteenth Knowledge Sharing Article (KSA) on standardized methods

November 7, 2013

KSA-SM-14: Using R to calculate confidence intervals for the repeatability and reproducibility standard deviations and the intra-laboratory correlation coefficient of a disinfectant test method

Now available: Fifteenth Knowledge Sharing Article (KSA) on standardized methods

March 24, 2015